Silva L D, Onclin K, Lejeune B, Verstegen J P
Department of Small Animal Reproduction Veterinary College, University of Liège, Belgium.
Vet Rec. 1996 Feb 17;138(7):154-7. doi: 10.1136/vr.138.7.154.
To compare the importance of the route of insemination when using fresh or frozen semen, six groups of five bitches were inseminated either into the uterus (groups 4, 5 and 6) or the vagina (groups 1, 2 and 3) with fresh (groups 1 and 4) or frozen semen (groups 2, 3, 5 and 6). The fresh semen was collected when needed from the same dog. The frozen semen used in groups 2 and 5 was obtained from seven dogs on the same day, and pooled and processed simultaneously so that the groups were inseminated with exactly the same semen. The frozen semen used in groups 3 and 6 was obtained from different dogs and processed independently to evaluate not only the effect of the route of insemination but also the potential effect of the dog. The mean concentration of the fresh semen was 310 x 10(6) spermatozoa/ml, its motility was greater than 80 per cent and the percentage of normal live spermatozoa was 80 to 92 per cent. The mean spermatozoal concentration of the frozen semen was 200 x 10(6) spermatozoa/ml, its motility was greater than 60 per cent and the percentage of normal live spermatozoa was 80 per cent. In all the groups there were fewer than 15 per cent abnormal spermatozoa. The animals inseminated with fresh semen received significantly more spermatozoa than the others. The bitches were inseminated twice, three and five days after the estimated peak of luteinising hormone, with a total volume of 5 ml for the vaginal inseminations and 2 ml for the intrauterine inseminations. Sixty per cent of the bitches inseminated with frozen semen and 100 per cent of the bitches inseminated with fresh semen became pregnant, irrespective of the insemination technique used.
为比较使用新鲜或冷冻精液时授精途径的重要性，将六组每组五只母犬分别经子宫（第4、5和6组）或阴道（第1、2和3组）进行授精，其中第1和4组使用新鲜精液，第2、3、5和6组使用冷冻精液。新鲜精液在需要时从同一只犬采集。第2和5组使用的冷冻精液于同一天从七只犬采集，混合并同时处理，以使两组授精时使用完全相同的精液。第3和6组使用的冷冻精液从不同犬采集并独立处理，以不仅评估授精途径的影响，还评估犬的潜在影响。新鲜精液的平均浓度为3.1×10⁷精子/ml，其活力大于80%，正常活精子百分比为80%至92%。冷冻精液的平均精子浓度为2.0×10⁷精子/ml，其活力大于60%，正常活精子百分比为80%。所有组中异常精子均少于15%。用新鲜精液授精的动物比其他动物接受的精子明显更多。母犬在估计的促黄体生成素峰值后2、3和5天进行两次授精，阴道授精总量为5 ml，子宫内授精总量为2 ml。无论采用何种授精技术，60%用冷冻精液授精的母犬和100%用新鲜精液授精的母犬怀孕。
