Wang Hengshan, Zhao Shulin, He Min, Zhao Zhicai, Pan Yingming, Liang Qian
College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, China.
J Sep Sci. 2007 Nov;30(16):2748-53. doi: 10.1002/jssc.200700128.
A new type of chiral surfactant, sodium maleopimaric acid (SMA), was synthesized, and employed for the enantioselective micellar electrokinetic chromatographic (MEKC) separation of amino acid enantiomers derivatized with naphthalene-2,3-dicarboxaldehyde (NDA-D/L-AAs). The effect of the surfactant concentration, type and concentration of the BGE, and buffer pH on the resolution was studied, and optimized conditions were used to evaluate the ability of this new surfactant to perform chiral separations toward NDA-D/L-AAs by MEKC. Enantiomeric separations of NDA-D/L-AAs were achieved with a running buffer consisting of 100 mM borate (pH 9.5) and 20 mM SMA in a 58.5 cm length x 50 microm id capillary. Under the conditions selected, two pairs of tested amino acid enantiomers including NDA-D/L-trptophan (Trp) and NDA-D/L-kynurenine (Kyn) were resolved.
合成了一种新型手性表面活性剂马来海松酸钠（SMA），并将其用于萘-2,3-二甲醛（NDA-D/L-氨基酸）衍生化的氨基酸对映体的对映选择性胶束电动色谱（MEKC）分离。研究了表面活性剂浓度、背景电解质（BGE）的类型和浓度以及缓冲液pH对分离度的影响，并使用优化条件评估这种新型表面活性剂通过MEKC对NDA-D/L-氨基酸进行手性分离的能力。在一根长度为58.5 cm、内径为50 μm的毛细管中，采用由100 mM硼酸盐（pH 9.5）和20 mM SMA组成的运行缓冲液实现了NDA-D/L-氨基酸的对映体分离。在所选择的条件下，分离出了两对测试氨基酸对映体，包括NDA-D/L-色氨酸（Trp）和NDA-D/L-犬尿氨酸（Kyn）。
