Camilleri P
SmithKline Beecham Pharmaceuticals, Harlow, Essex, UK.
Electrophoresis. 1997 Nov;18(12-13):2322-30. doi: 10.1002/elps.1150181223.
Most biologically active molecules contain one or more chiral centres, giving rise to stereoisomeric forms which can behave differently in a chiral environment. Thus, only one of the enantiomers may show the desired physiological activity, whereas the other enantiomers may either be considerably less active or even show undesireable side effects. Establishing that a chiral drug consists of one single enantiomer is nowadays essential before it can be given to patients. Analytical tools that can discriminate between enantiomers play a very important role in determining the stereoisomeric composition of chiral molecules. Until recently chromatographic techniques were the most popular for enantiomeric separations. The increased use of capillary electrophoresis (CE) has provided complementary methodology for chiral discrimination. One mode of CE that has been used for this purpose is micellar electrokinetic capillary chromatography (MECC), where natural or synthetic chiral surfactants are added to the separation buffer.
大多数生物活性分子含有一个或多个手性中心，会产生立体异构体形式，这些立体异构体在 chiral 环境中的行为可能不同。因此，只有一种对映体可能表现出所需的生理活性，而其他对映体可能活性大大降低，甚至表现出不良副作用。如今，在将手性药物给予患者之前，确定其由单一的对映体组成至关重要。能够区分对映体的分析工具在确定手性分子的立体异构体组成方面起着非常重要的作用。直到最近，色谱技术仍是对映体分离最常用的方法。毛细管电泳（CE）的使用增加为手性鉴别提供了补充方法。用于此目的的一种 CE 模式是胶束电动毛细管色谱（MECC），其中将天然或合成的手性表面活性剂添加到分离缓冲液中。
