大鼠肝脏中二氢嘧啶酶编码cDNA的分子克隆与测序

Molecular cloning and sequencing of a cDNA encoding dihydropyrimidinase from the rat liver.

作者信息

Matsuda K, Sakata S, Kaneko M, Hamajima N, Nonaka M, Sasaki M, Tamaki N

机构信息

Faculty of Nutrition, Kobe Gakuin University, Japan.

出版信息

Biochim Biophys Acta. 1996 Jun 7;1307(2):140-4. doi: 10.1016/0167-4781(96)00056-5.

DOI:10.1016/0167-4781(96)00056-5

PMID:8679696

Abstract

A cDNA encoding dihydropyrimidinase has been isolated from a rat cDNA library. The N-terminal and an internal amino acid sequences were determined, and PCR primers were designed based on these sequences. Using a cDNA fragment amplified by RT-PCR with these primers, three cDNA clones were isolated from a rat liver library. The clone with the longest insert of 2129 bp contained a 1557 bp open reading frame encoding a polypeptide of 519 residues with a molecular mass of 56,832 Da.

摘要

已从大鼠cDNA文库中分离出编码二氢嘧啶酶的cDNA。测定了其N端和内部氨基酸序列，并根据这些序列设计了PCR引物。使用这些引物通过RT-PCR扩增的cDNA片段，从大鼠肝脏文库中分离出三个cDNA克隆。插入片段最长为2129 bp的克隆包含一个1557 bp的开放阅读框，编码一个由519个残基组成、分子量为56,832 Da的多肽。