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编码人肌肉型肉碱棕榈酰转移酶I的cDNA和基因组克隆的分离与鉴定

Isolation and characterization of cDNA and genomic clones encoding human muscle type carnitine palmitoyltransferase I.

作者信息

Yamazaki N, Shinohara Y, Shima A, Yamanaka Y, Terada H

机构信息

Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.

出版信息

Biochim Biophys Acta. 1996 Jun 7;1307(2):157-61. doi: 10.1016/0167-4781(96)00069-3.

DOI:10.1016/0167-4781(96)00069-3
PMID:8679700
Abstract

With a cDNA probe encoding rat muscle type carnitine palmitoyltransferase I (CPTI), we isolated cDNA and genomic clones encoding the human homologue and deduced the primary structure of human muscle type CPTI. By Northern analysis, we confirmed the dominant expression of this isoform in heart and skeletal muscle.

摘要

利用编码大鼠肌肉型肉碱棕榈酰转移酶I(CPTI)的cDNA探针,我们分离出了编码人同源物的cDNA和基因组克隆,并推导了人肌肉型CPTI的一级结构。通过Northern分析,我们证实了这种同工型在心脏和骨骼肌中的优势表达。

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