Yamazaki N, Shinohara Y, Shima A, Terada H
Faculty of Pharmaceutical Sciences, University of Tokushima, Japan.
FEBS Lett. 1995 Apr 17;363(1-2):41-5. doi: 10.1016/0014-5793(95)00277-g.
To characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted amino acid sequence showed the highest homology (62.6%) with that of rat carnitine palmitoyltransferase I (CPTI). The transcript corresponding to this cDNA was found to be abundantly expressed in BAT and heart. Therefore, the isolated clone is concluded to encode a CPTI like protein expressed in BAT and heart.
为了表征大鼠棕色脂肪组织（BAT）中的能量代谢，我们用白色脂肪组织（WAT）的cDNA探针进行BAT cDNA文库的差异筛选，并分离出一个cDNA克隆。它包含一个2316个碱基的单一开放阅读框，编码一个88.2 kDa的蛋白质。预测的氨基酸序列与大鼠肉碱棕榈酰转移酶I（CPTI）的氨基酸序列具有最高的同源性（62.6%）。发现与该cDNA对应的转录本在BAT和心脏中大量表达。因此，得出结论，分离出的克隆编码一种在BAT和心脏中表达的CPTI样蛋白。
