Jahnson S, Gerdin B
Department of Urology, Orebro Medical Centre, Sweden.
Eur J Surg. 1996 Jan;162(1):47-53.
To assess the influence of protein malnutrition on anastomotic healing in rat small bowel with or without chronic radiation damage.
Controlled laboratory study.
University hospital, Sweden.
60 male Sprague-Dawley rats.
A short segment of the distal ileum was exteriorised and irradiated (n = 30) or only exposed (n = 30), and 20 weeks later an anastomosis was made within this segment. Two weeks before anastomosis half of the animals in each group received rat chow in which the protein content had been reduced to 25%; standard rat chow was given to the remaining animals.
Weight changes, anastomotic bursting strength, amount of perianastomotic hydroxyproline, and number of anastomotic complications.
13 animals in the irradiated group and 11 animals in the non-irradiated group died of intestinal obstruction or respiratory distress leaving 17 and 19 animals that could be evaluated. Body weight was significantly reduced in animals with protein restriction (p < 0.001). A two way ANOVA showed an association between bursting strength and irradiation (p = 0.02) but not bursting strength and protein restriction. Anastomotic complications were more common in irradiated than in non-irradiated animals irrespective of the nutrition given (8/8 and 8/9 compared with 2/9 and 2/10, p = 0.0006).
Protein malnutrition had no influence on anastomotic healing in rat intestine with or without chronic radiation damage.
评估蛋白质营养不良对有或无慢性辐射损伤的大鼠小肠吻合口愈合的影响。
对照实验室研究。
瑞典的大学医院。
60只雄性斯普拉格 - 道利大鼠。
将一段远端回肠外置，其中30只进行照射，30只仅暴露，20周后在该段肠管内进行吻合术。在吻合术前两周，每组一半的动物给予蛋白质含量降至25%的大鼠饲料；其余动物给予标准大鼠饲料。
体重变化、吻合口破裂强度、吻合口周围羟脯氨酸含量及吻合口并发症数量。
照射组13只动物和未照射组11只动物死于肠梗阻或呼吸窘迫，剩余17只和19只动物可供评估。蛋白质限制组动物体重显著降低（p < 0.001）。双向方差分析显示破裂强度与照射有关（p = 0.02），但与蛋白质限制无关。无论给予何种营养，照射动物的吻合口并发症比未照射动物更常见（8/8和8/9与2/9和2/10相比，p = 0.0006）。
蛋白质营养不良对有或无慢性辐射损伤的大鼠肠道吻合口愈合无影响。
