Glavac D, Dean M
National Institute of Chemistry, Ljubljana, Slovenia.
Hum Mutat. 1995;6(4):281-7. doi: 10.1002/humu.1380060402.
Double-stranded heteroduplex molecules that form between a mutant and wild-type DNA strand are often distinguished from homoduplex molecules upon gel electrophoresis. This method, heteroduplex analysis (HA), can be performed rapidly without radioisotopes or specialized equipment. Modifications and enhancements of the HA method have been developed that increase the sensitivity of detection of single-base pair alterations.
在凝胶电泳时，突变型DNA链与野生型DNA链之间形成的双链异源双链分子通常能与同源双链分子区分开来。这种方法，即异源双链分析（HA），无需使用放射性同位素或专门设备就能快速完成。人们已经对HA方法进行了改进和优化，提高了对单碱基对改变的检测灵敏度。
