Nataraj A J, Olivos-Glander I, Kusukawa N, Highsmith W E
FMC BioProducts, Rockland, ME, USA.
Electrophoresis. 1999 Jun;20(6):1177-85. doi: 10.1002/(SICI)1522-2683(19990101)20:6<1177::AID-ELPS1177>3.0.CO;2-2.
Single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) are popular electrophoretic methods for the identification of sequences. The principle reasons for the popularity of these two methods are their technical simplicity and their relatively high sensitivity for the detection of mutations. Here we review the theory and practice of SSCP and HA, including the factors contributing to the sensitivity of mutation detection. For SSCP analysis, these factors include: choice of gel matrix, electrophoretic conditions, presence of neutral additives, fragment size, and G+C content For HA, the principle factors influencing sensitivity are the gel matrix and the identity of the base mismatch.
单链构象多态性（SSCP）和异源双链分析（HA）是用于序列鉴定的常用电泳方法。这两种方法广受欢迎的主要原因在于其技术操作简单，且对突变检测具有相对较高的灵敏度。在此，我们综述了SSCP和HA的理论与实践，包括影响突变检测灵敏度的因素。对于SSCP分析，这些因素包括：凝胶基质的选择、电泳条件、中性添加剂的存在、片段大小以及G+C含量。对于HA，影响灵敏度的主要因素是凝胶基质和碱基错配的情况。
