同时高效液相色谱法测定玉米上镰刀菌培养物中的维索曲菌素、尖顶吡喃酮和厚垣孢子醇。

Simultaneous high-performance liquid chromatographic determination of visoltricin, acuminatopyrone and chlamydosporols in Fusarium cultures on maize.

作者信息

Solfrizzo M, Visconti A

机构信息

Istituto Tossine e Micotossine da Parassiti Vegetali, CNR, Bari, Italy.

出版信息

J Chromatogr A. 1996 Apr 12;730(1-2):69-73. doi: 10.1016/0021-9673(95)00899-3.

DOI:10.1016/0021-9673(95)00899-3

PMID:8680598

Abstract

Visoltricin (VIS), acuminatopyrone (ACP), clamydosporol (CL), isochlamydosporol (ICL) and chlamydospordiol (DIOL), recently characterized Fusarium metabolites, were separated on a polymeric RP-18 column eluted with acetonitrile-0.01% ammonia solution (35:65) at 1 ml/min and detected with a diode-array UV detector. The presence of ammonia in the mobile phase improved the shape of the CL and VIS peaks. The use of a polymeric column was required owing to the basic pH of the mobile phase. Maize cultures of several strains of F.tricinctum and F. chlamydosporum were analysed with this procedure after extraction with aqueous methanol, partitioning with methylene chloride and clean-up with a C18 minicolumn. VIS was produced only by F. tricinctum, whereas ACP and chlamydosporols were produced by both Fusarium species.

摘要

维索曲菌素（VIS）、尖孢吡喃酮（ACP）、枝孢醇（CL）、异枝孢醇（ICL）和枝孢二醇（DIOL）是最近鉴定出的镰刀菌代谢产物，它们在聚合物反相18柱上分离，以乙腈-0.01%氨溶液（35:65）为流动相，流速为1 ml/min，用二极管阵列紫外检测器检测。流动相中氨的存在改善了CL和VIS峰的峰形。由于流动相的碱性pH值，需要使用聚合物柱。在用甲醇水溶液萃取、二氯甲烷分配和C18微柱净化后，用此方法分析了几种三线镰刀菌和厚垣孢子镰刀菌菌株的玉米培养物。VIS仅由三线镰刀菌产生，而ACP和枝孢醇则由这两种镰刀菌产生。