Jiménez M, Mateo R
Department of Microbiology, University of Valencia, Spain.
J Chromatogr A. 1997 Aug 22;778(1-2):363-72. doi: 10.1016/s0021-9673(97)00328-2.
A method of analysis for trichothecenes (nivalenol, deoxynivalenol, 3- and 15-acetyldeoxynivalenol, diacetoxyscirpenol, neosolaniol, T-2 tetraol, T-2 and HT-2 toxins), zearalenone and zearalenols, and another method for determination of fumonisin B1 are described and applied to cultures of Fusarium isolated from bananas. Both methods were adapted from different techniques of extraction, clean-up and determination of these mycotoxins. The first method involves extraction with methanol-1% aqueous sodium chloride, clean-up of extracts by partition with hexane and dichloromethane, additional solid reversed-phase clean-up and analysis of two eluates by both high-performance liquid chromatography with ultraviolet detection and capillary gas chromatography. The method for fumonisin B1 implies extraction with aqueous methanol, concentration, clean-up with water and methanol on Amberlite XAD-2 column, formation of a fluorescent 4-fluoro-7-nitrobenzofurazan derivative and analysis by high-performance liquid chromatography with fluorescence detection. Both procedures give good limits of detection and recoveries, and are considered suitable for the detection and quantification of the studied toxins in corn and rice cultures of Fusarium spp. isolated from banana fruits.
本文描述了一种分析单端孢霉烯族毒素（雪腐镰刀菌烯醇、脱氧雪腐镰刀菌烯醇、3-和15-乙酰脱氧雪腐镰刀菌烯醇、二醋酸藨草镰刀菌烯醇、新茄病镰刀菌烯醇、T-2四醇、T-2和HT-2毒素）、玉米赤霉烯酮和玉米赤霉烯醇的方法，以及另一种测定伏马菌素B1的方法，并将其应用于从香蕉中分离出的镰刀菌培养物。这两种方法均改编自这些霉菌毒素不同的提取、净化和测定技术。第一种方法包括用甲醇-1%氯化钠水溶液提取，通过用己烷和二氯甲烷分配来净化提取物，额外的固相反相净化，以及通过高效液相色谱-紫外检测和毛细管气相色谱对两种洗脱液进行分析。伏马菌素B1的测定方法包括用水性甲醇提取、浓缩、在Amberlite XAD-2柱上用水和甲醇净化，并形成荧光4-氟-7-硝基苯并呋喃唑衍生物，然后通过高效液相色谱-荧光检测进行分析。这两种方法都具有良好的检测限和回收率，并且被认为适用于检测和定量从香蕉果实中分离出的镰刀菌在玉米和水稻培养物中所研究的毒素。
