Perocco P, Colacci A, Del Ciello C, Grilli S
Istituto di Cancerologia, Università di Bologna, Italy.
Res Commun Mol Pathol Pharmacol. 1995 Sep;89(3):329-39.
Further information was gathered on the possible carcinogenic hazard associated to the exposure to the insecticide lindane (gamma-hexachlorocyclohexane). The parameters studied were the cytotoxic and cell transforming activities of the pesticide on BALB/c 3T3 cells in an in vitro experimental model system in the absence or in the presence of rat liver S-9 mix-induced metabolic activation of the chemical. Lindane did not exert cytotoxic effects at all the tested doses (ranging from 10 micrograms/ml to 200 micrograms/ml) in the absence of bioactivation. However, dose-related cytotoxic effects were observed in the presence of the metabolizing system. Furthermore, lindane showed statistically significant and dose-dependent cell transformation activity at all the tested doses (10 micrograms/ml, 50 micrograms/ml and 100 micrograms/ml ) either in the absence or in the presence of bioactivation. This activity was related with cell proliferation since it was exerted in a level-II transformation test by replating cells and allowing the amplification of the cell transforming effects of the chemical. The formation of radicals and of reactive oxygen species, resulting from the chemical metabolism, could account for lindane activity as carcinogenesis promoting agent, although contemporary genotoxic effects induced by the pesticide could not be excluded.
收集了更多关于接触杀虫剂林丹（γ-六氯环己烷）可能存在的致癌风险的信息。所研究的参数包括在体外实验模型系统中，在不存在或存在大鼠肝脏S-9混合物诱导的该化学物质代谢活化的情况下，该农药对BALB/c 3T3细胞的细胞毒性和细胞转化活性。在没有生物活化的情况下，林丹在所有测试剂量（范围从10微克/毫升至200微克/毫升）下均未产生细胞毒性作用。然而，在存在代谢系统的情况下观察到了剂量相关的细胞毒性作用。此外，无论是否存在生物活化，林丹在所有测试剂量（10微克/毫升、50微克/毫升和100微克/毫升）下均表现出具有统计学意义的剂量依赖性细胞转化活性。这种活性与细胞增殖有关，因为它是在二级转化试验中通过重新接种细胞并使该化学物质的细胞转化作用得以放大而发挥作用的。化学物质代谢产生的自由基和活性氧物种的形成，可能是林丹作为致癌促进剂发挥活性的原因，尽管不能排除该农药同时诱导的遗传毒性作用。
