Perocco P, Colacci A, Santucci M A, Vaccari M, Grilli S
Centro Interuniversitario per la Ricerca sul Cancro, Universita' di Bologna, Italy.
Res Commun Chem Pathol Pharmacol. 1991 Aug;73(2):159-72.
Ethylene dibromide was capable of inducing in vitro transformation of BALB/c 3T3 cells either in the presence or in the absence of exogenous metabolic activation (S9-mix). This transforming effect was evidenced by the induction of a higher number of transformed foci as compared to the controls performed with untreated cells or solvent vehicle-treated cells. In the absence of exogenous activation, all assayed doses (ranging from 23.4 micrograms/ml to 187.9 micrograms/ml) exerted transforming activity. Number of foci obtained in EDB-treated plates antransformation frequency of the target cells were higher than those detected in the transformation test performed in the presence of S9-mix.
在有或没有外源性代谢活化（S9混合物）的情况下，二溴乙烷都能够诱导BALB/c 3T3细胞发生体外转化。与用未处理细胞或溶剂处理细胞进行的对照相比，诱导出更多的转化灶证明了这种转化作用。在没有外源性活化的情况下，所有测定剂量（范围从23.4微克/毫升至187.9微克/毫升）都具有转化活性。在二溴乙烷处理的平板上获得的灶数和靶细胞的转化频率高于在有S9混合物存在下进行的转化试验中检测到的数值。
