腐殖酸对家兔肝细胞线粒体代谢抑制作用的微量量热研究
Microcalorimetric study of metabolic inhibition by humic acids in mitochondria from Oryctolagus cuniculus domestica liver cells.
作者信息
Liu Y, Wang X Q, Xie C L, Qu S S, Deng F J, Guo Y
机构信息
Department of Chemistry, Wuhan University, P.R. China.
出版信息
Chemosphere. 1996 Jul;33(1):99-105. doi: 10.1016/0045-6535(96)00151-8.
The metabolic thermogenesis curves of mitochondria isolated from Oryctolagus cuniculus domestica liver tissue have been determined by using an LKB-2277 bioactivity monitor. After isolation, mitochondria maintain activity when stored in appropriate nutrients. The thermokinetic equation: [formula: see text] was established for the activity recovery phase of metabolism. For these studies, the order of metabolism was found to be zero. The metabolic activity of mitochondria was inhibited by humic acid, a potential toxicant in Kashin-Beck and Keshan diseases. Experimental results indicate that humic acid will effect the heat output and lag time of mitochondrial metabolism.
利用LKB - 2277生物活性监测仪测定了从家兔肝脏组织分离出的线粒体的代谢产热曲线。分离后，线粒体在储存于适当营养物质时能保持活性。为代谢的活性恢复阶段建立了热动力学方程：[公式：见原文]。对于这些研究，发现代谢顺序为零级。线粒体的代谢活性受到腐殖酸的抑制，腐殖酸是大骨节病和克山病中的一种潜在毒物。实验结果表明，腐殖酸会影响线粒体代谢的热输出和延迟时间。
Zotero 插件