从鱼肝组织分离的线粒体的微量量热研究。

Microcalorimetric study of mitochondria isolated from fish liver tissue.

作者信息

Tan A M, Xie C L, Qu S S

机构信息

Department of Chemistry, Wuhan University, People's Republic of China.

出版信息

J Biochem Biophys Methods. 1996 Feb 5;31(3-4):189-93. doi: 10.1016/0165-022x(95)00034-o.

DOI:10.1016/0165-022x(95)00034-o

PMID:8675962

Abstract

We determined the thermogenesis curves of mitochondria isolated from fish liver tissue by using an LKB 2277 Bioactivity Monitor. After isolation from the fish liver, mitochondria still have activity and can live for a long time by using the stored nutrients. We calculated the recovery rate constants of mitochondria. We found that the thermogenesis curves of mitochondria are similar to those obtained from prokaryotic cells, but not similar to those obtained from eukaryotic cells. We determined the metabolic thermogenesis curves of mitochondria isolated from two kinds of carp liver tissue, scattered-scaled mirror carp and harvest carp. There are some important similarities and some important differences between these thermogenesis curves.

摘要

我们使用LKB 2277生物活性监测仪测定了从鱼肝组织分离出的线粒体的产热曲线。从鱼肝中分离出来后，线粒体仍具有活性，并且可以通过利用储存的营养物质存活很长时间。我们计算了线粒体的恢复速率常数。我们发现线粒体的产热曲线与从原核细胞获得的产热曲线相似，但与从真核细胞获得的产热曲线不同。我们测定了从两种鲤鱼肝脏组织（散鳞镜鲤和丰鲤）分离出的线粒体的代谢产热曲线。这些产热曲线之间存在一些重要的相似之处和一些重要的差异。