Netzel B, Bornkamm G, Lenhard V, Scholz S, Grosse-Wilde H
Z Immunitatsforsch Immunobiol. 1977 Apr;153(1):85-9.
Lymphoblastoid cell lines (LCL) was established from peripheral blood lymphocytes (PBL) of 2 HLA-DW 3 homozygous siblings. HLA-D typing was performed with the homozygous LCL and PBL in a group of 51 unrelated individuals selected according to their HLA-A,B locus antigens. All the individuals who were typed positive for DW3 with PBL typing cells could also be typed positive with the LCL typing cells. Both homozygous LCL behave very similarly in the tested population. These experiments showed that the same HLA-D determinants were represented both on LCL and PBL cells and that LCL derived from MLC homozygous donors can be used for HLA-D typing.
从2名HLA - DW3纯合子同胞的外周血淋巴细胞（PBL）建立了淋巴母细胞系（LCL）。在一组根据其HLA - A、B位点抗原选择的51名无关个体中，用纯合LCL和PBL进行HLA - D分型。所有用PBL分型细胞检测为DW3阳性的个体，用LCL分型细胞检测也为阳性。在受试群体中，两种纯合LCL的表现非常相似。这些实验表明，LCL和PBL细胞上都表达相同的HLA - D决定簇，并且来自混合淋巴细胞培养（MLC）纯合供体的LCL可用于HLA - D分型。
