Akiyama N, Ohsawa N, Samaru Y, Kusaba R
Jpn J Exp Med. 1982 Apr;52(2):67-73.
We examined whether Epstein-Barr virus-induced lymphoblastoid cell lines, established from homozygous typing cells (HTC-EBV-LCL), could be substituted for normal homozygous typing cells (HTC) in HLA-D typing. When using EBV-LCL as stimulators in one-way MLR, the stimulator: responder ratio was found to be most important. At a ratio of 1:10, the autologous MLR between EBV-LCL and PBL from the same donors exhibited low double normalized values (DNV) which could be distinguished from those of homologous combinations. The panel cells typed with HTC always exhibited low DNV in repeat one-way MLR in which EBV-LCL established from the same HTC were used. On the other hand, by employing our previously described method, we were able to establish the necessary HTC-EBV-LCL from unselected seropositive adult donors and utilize these cells for HLA-D typing. We suggest that our method makes it possible to circumvent the problem presented by the shortage of HTC, since our HTC-EBV-LCL can be substituted for normal HTC in HLA-D typing.
我们研究了从纯合分型细胞（HTC-EBV-LCL）建立的爱泼斯坦-巴尔病毒诱导的淋巴母细胞系是否可在HLA-D分型中替代正常纯合分型细胞（HTC）。当在单向混合淋巴细胞反应（MLR）中使用EBV-LCL作为刺激细胞时，发现刺激细胞与反应细胞的比例最为重要。在1:10的比例下，来自相同供体的EBV-LCL与外周血淋巴细胞（PBL）之间的自体MLR表现出较低的双标准化值（DNV），这可与同源组合的双标准化值区分开来。在用HTC分型的一组细胞中，在重复的单向MLR中（其中使用从相同HTC建立的EBV-LCL）总是表现出较低的DNV。另一方面，通过采用我们先前描述的方法，我们能够从未经选择的血清学阳性成年供体中建立所需的HTC-EBV-LCL，并将这些细胞用于HLA-D分型。我们认为我们的方法使得有可能规避HTC短缺所带来的问题，因为我们的HTC-EBV-LCL可在HLA-D分型中替代正常的HTC。
