Valenzuela J G, Guimaraes J A, Ribeiro J M
Department of Entomology, University of Arizona, Tucson 85721, USA.
Exp Parasitol. 1996 Jul;83(2):184-90. doi: 10.1006/expr.1996.0065.
Cimex lectularius salivary gland homogenate delayed the recalcification time of human citrated plasma. Separation of the salivary gland homogenate by molecular sieving HPLC chromatography resulted in a single major peak of anticlotting activity with an apparent molecular mass of 17,000. The anticoagulant principle inhibited the activation of factor X to factor Xa in the tenase complex (FVIII, FIXa, FX, phospholipids, and calcium). However, it did not directly inhibit already activated factor Xa, suggesting that the anticlotting activity is not an anti-factor Xa. Additionally, this salivary gland anticoagulant further retarded the recalcification time of factor VIII- and factor IX-deficient plasmas, suggesting that the anticlotting principle is not directly inhibiting either the coagulation factor VIII or factor IXa. Altogether these data suggest that the anticlotting activity is an inhibitor of the activation of factor X to factor Xa in the tenase complex.
温带臭虫唾液腺匀浆可延长人枸橼酸盐血浆的再钙化时间。通过分子筛分高效液相色谱法分离唾液腺匀浆，得到一个抗凝血活性的主要峰，其表观分子量为17,000。抗凝原理是抑制凝血酶原酶复合物（FVIII、FIXa、FX、磷脂和钙）中因子X激活为因子Xa。然而，它并不直接抑制已激活的因子Xa，这表明抗凝血活性不是抗因子Xa。此外，这种唾液腺抗凝剂进一步延长了因子VIII和因子IX缺乏血浆的再钙化时间，这表明抗凝原理并不直接抑制凝血因子VIII或因子IXa。总之，这些数据表明抗凝血活性是凝血酶原酶复合物中因子X激活为因子Xa的抑制剂。
