Weterman M A, Wilbrink M, Dijkhuizen T, van den Berg E, Geurts van Kessel A
Department of Human Genetics, University Hospital Nijmegen, The Netherlands.
Hum Genet. 1996 Jul;98(1):16-21. doi: 10.1007/s004390050153.
A combination of Southern blot analysis on a panel of tumor-derived somatic cell hybrids and fluorescence in situ hybridization (FISH) techniques was used to map a series of DNA markers relative to the 1q21 breakpoint of the renal cell carcinoma (RCC)-associated (X;1)-(p11;q21) translocation. This breakpoint maps between several members of the S100 family which are clustered in the 1q21 region and a conserved region between man and mouse containing the markers SPTA1-CRP-APCS-FcER1A-ATP1A2-APOA2. The location of the breakpoint coincides with the transition of a region of synteny of human chromosome 1 with mouse chromosomes 3 and 1.
利用一组肿瘤来源的体细胞杂种进行Southern印迹分析，并结合荧光原位杂交（FISH）技术，来绘制一系列与肾细胞癌（RCC）相关的(X;1)-(p11;q21)易位的1q21断点相关的DNA标记。该断点定位于S100家族的几个成员之间，这些成员聚集在1q21区域，以及人与小鼠之间的一个保守区域，该保守区域包含标记SPTA1-CRP-APCS-FcER1A-ATP1A2-APOA2。断点的位置与人1号染色体与小鼠3号和1号染色体的同线性区域的转变相吻合。
