Kaufman R I, Nixon B T
Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania 16802, USA.
J Bacteriol. 1996 Jul;178(13):3967-70. doi: 10.1128/jb.178.13.3967-3970.1996.
Degenerate PCR probes were used to amplify gene fragments encoding the catalytic domain of sigma54-dependent transcription activators. The procedure should be widely applicable, as it recovered both known and novel gene fragments: 5 from Rhizobium meliloti, 13 from Myxococcus xanthus, and 3 from Bacillus subtilis. No fragments were obtained from Synechococcus sp. strain PCC 7002 or Saccharomyces cerevisiae.
简并PCR探针用于扩增编码σ54依赖性转录激活因子催化结构域的基因片段。该方法应具有广泛的适用性，因为它既能回收已知基因片段，也能回收新的基因片段：来自苜蓿根瘤菌的有5个，来自黄色粘球菌的有13个，来自枯草芽孢杆菌的有3个。未从聚球藻属菌株PCC 7002或酿酒酵母中获得片段。
