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苜蓿根瘤菌DctD（一种σ54依赖性激活因子）磷酸结合环的突变分析

Mutational analysis of the phosphate-binding loop of Rhizobium meliloti DctD, a sigma54-dependent activator.

作者信息

Gao Y, Wang Y K, Hoover T R

机构信息

Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.

出版信息

J Bacteriol. 1998 May;180(10):2792-5. doi: 10.1128/JB.180.10.2792-2795.1998.

DOI:10.1128/JB.180.10.2792-2795.1998
PMID:9573172
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC107239/
Abstract

The phosphate-binding loop of sigma54-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in Rhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.

摘要

σ54 依赖性激活因子的磷酸结合环被认为参与 ATP 的结合和/或水解。苜蓿根瘤菌 DctD 中该基序第 3、4、6、7 和 8 位的丙氨酸取代破坏了转录激活和 ATP 水解。有趣的是，第 7 位丙氨酸的取代也影响了 DNA 结合。

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本文引用的文献

1
A conserved region in the sigma54-dependent activator DctD is involved in both binding to RNA polymerase and coupling ATP hydrolysis to activation.依赖σ54的激活因子DctD中的一个保守区域既参与与RNA聚合酶的结合，又参与将ATP水解与激活偶联起来。
Mol Microbiol. 1997 Oct;26(2):373-86. doi: 10.1046/j.1365-2958.1997.5851955.x.
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Alterations within the activation domain of the sigma 54-dependent activator DctD that prevent transcriptional activation.σ54依赖型激活因子DctD的激活结构域内发生的改变，这些改变会阻止转录激活。
J Bacteriol. 1997 Sep;179(18):5812-9. doi: 10.1128/jb.179.18.5812-5819.1997.
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Transcriptional activation via DNA-looping: visualization of intermediates in the activation pathway of E. coli RNA polymerase x sigma 54 holoenzyme by scanning force microscopy.通过DNA环化实现转录激活：利用扫描力显微镜观察大肠杆菌RNA聚合酶x σ54全酶激活途径中的中间体
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Cooperative binding of DctD to the dctA upstream activation sequence of Rhizobium meliloti is enhanced in a constitutively active truncated mutant.在一个组成型激活的截短突变体中，苜蓿根瘤菌的DctD与dctA上游激活序列的协同结合增强。
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Repressor forms of the enhancer-binding protein NrtC: some fail in coupling ATP hydrolysis to open complex formation by sigma 54-holoenzyme.增强子结合蛋白NrtC的阻遏物形式：一些在将ATP水解与σ54-全酶形成开放复合物相偶联方面失败。
J Mol Biol. 1996 Jul 19;260(3):317-31. doi: 10.1006/jmbi.1996.0403.
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ATP binding to the sigma 54-dependent activator XylR triggers a protein multimerization cycle catalyzed by UAS DNA.ATP与依赖于σ54的激活因子XylR结合，触发了由UAS DNA催化的蛋白质多聚化循环。
Cell. 1996 Jul 26;86(2):331-9. doi: 10.1016/s0092-8674(00)80104-x.
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Activity of purified NIFA, a transcriptional activator of nitrogen fixation genes.纯化的NIFA（一种固氮基因的转录激活因子）的活性。
Proc Natl Acad Sci U S A. 1993 Mar 15;90(6):2266-70. doi: 10.1073/pnas.90.6.2266.
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Primary structural constraints of P-loop of mitochondrial F1-ATPase from yeast.酵母线粒体F1-ATP酶P环的一级结构限制因素
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Constitutive ATP hydrolysis and transcription activation by a stable, truncated form of Rhizobium meliloti DCTD, a sigma 54-dependent transcriptional activator.由苜蓿根瘤菌DCTD的稳定截短形式（一种依赖σ54的转录激活因子）介导的组成型ATP水解和转录激活。
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