苜蓿根瘤菌DctD（一种σ54依赖性激活因子）磷酸结合环的突变分析
Mutational analysis of the phosphate-binding loop of Rhizobium meliloti DctD, a sigma54-dependent activator.
作者信息
Gao Y, Wang Y K, Hoover T R
机构信息
Department of Microbiology, University of Georgia, Athens, Georgia 30602, USA.
出版信息
J Bacteriol. 1998 May;180(10):2792-5. doi: 10.1128/JB.180.10.2792-2795.1998.
Abstract
The phosphate-binding loop of sigma54-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in Rhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.
摘要
σ54 依赖性激活因子的磷酸结合环被认为参与 ATP 的结合和/或水解。苜蓿根瘤菌 DctD 中该基序第 3、4、6、7 和 8 位的丙氨酸取代破坏了转录激活和 ATP 水解。有趣的是，第 7 位丙氨酸的取代也影响了 DNA 结合。
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