Gu Y, Seidel A
Forschungszentrum Karlsruhe, Technik und Umwelt, Institut für Toxikologie, Germany.
Immunopharmacol Immunotoxicol. 1996 Feb;18(1):115-28. doi: 10.3109/08923979609007114.
The influence of the beta-adrenergic agonists Salbutamol and Isoproterenol on the release of reactive oxygen species and Tumor Necrosis Factor (TNF) was tested with bovine alveolar macrophages and HL-60 cells differentiated to macrophages by calcitriol. The production of reactive oxygen species was analyzed by a microplate assay using dichlorofluorescein-diacetate. It could be shown that this method almost exclusively measures superoxide anions. TNF was determined by a bioassay with WEHI cells. The superoxide anion production was stimulated by Zymosan, the TNF release by LPS. By incubation with 5 x 10(-6) and 5 x 10(-7) M Salbutamol or 5 x 10(-7) and 5 x 10(-8) M Isoproterenol prior to the stimulation, the production of superoxide anions as well as of TNF was inhibited to a significant degree. The inhibitory effects of the adrenergic agonists were completely or at least partially inhibited by the respective antagonists, ICI 118.551 and Propranolol, respectively.
使用牛肺泡巨噬细胞和经骨化三醇诱导分化为巨噬细胞的HL-60细胞，测试了β-肾上腺素能激动剂沙丁胺醇和异丙肾上腺素对活性氧和肿瘤坏死因子（TNF）释放的影响。使用二氯荧光素二乙酸酯通过微孔板测定法分析活性氧的产生。结果表明，该方法几乎仅检测超氧阴离子。TNF通过使用WEHI细胞的生物测定法测定。酵母聚糖刺激超氧阴离子的产生，脂多糖刺激TNF的释放。在刺激前，用5×10⁻⁶和5×10⁻⁷ M的沙丁胺醇或5×10⁻⁷和5×10⁻⁸ M的异丙肾上腺素孵育，超氧阴离子和TNF的产生均受到显著抑制。肾上腺素能激动剂的抑制作用分别被各自的拮抗剂ICI 118.551和普萘洛尔完全或至少部分抑制。
