Conlon P D, Ogunbiyi P O, Black W D, Eyre P
Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Canada.
Can J Physiol Pharmacol. 1988 Dec;66(12):1538-41. doi: 10.1139/y88-251.
Reactive oxygen species production by bovine pulmonary alveolar macrophages was evaluated by a chemiluminescence assay utilizing luminol and opsonized zymosan. Incubation with dobutamine (5 x 10(-8) and 5 x 10(-7) M) or isoproterenol (5 x 10(-8) and 5 x 10(-7) M) prior to zymosan challenge significantly (p less than 0.05) increased the time for chemiluminescence to begin, and significantly decreased the level of maximum chemiluminescence. The agonists' inhibitory effects on maximum chemiluminescence were significantly reduced by pre-incubation with the appropriate antagonist (atenolol at 1 x 10(-6) M for dobutamine; and propranolol at 1 x 10(-6) M for isoproterenol). Salbutamol at 1 x 10(-6) M significantly reduced the level of maximum chemiluminescence only, but did not increase the time for chemiluminescence to begin. This effect was significantly reduced by the presence of the beta 2-antagonist ICI 118,551 at 1 x 10(-6) M. The results reveal the presence of beta 1- and beta 2-adrenoceptors on bovine pulmonary alveolar macrophages, and suggest that these receptors are important in the regulation of reactive oxygen species production by these cells.
利用鲁米诺和调理酵母聚糖的化学发光分析法评估了牛肺泡巨噬细胞产生活性氧的情况。在酵母聚糖激发前，用多巴酚丁胺（5×10⁻⁸和5×10⁻⁷M）或异丙肾上腺素（5×10⁻⁸和5×10⁻⁷M）孵育，显著（p＜0.05）延长了化学发光开始的时间，并显著降低了最大化学发光水平。用合适的拮抗剂预孵育（多巴酚丁胺用1×10⁻⁶M的阿替洛尔；异丙肾上腺素用1×10⁻⁶M的普萘洛尔）可显著减轻激动剂对最大化学发光的抑制作用。1×10⁻⁶M的沙丁胺醇仅显著降低了最大化学发光水平，但未延长化学发光开始的时间。1×10⁻⁶M的β₂拮抗剂ICI 118,551可显著减轻这种作用。结果揭示了牛肺泡巨噬细胞上存在β₁和β₂肾上腺素能受体，并表明这些受体在调节这些细胞产生活性氧方面很重要。
