Lowings P, Ibata G, Needham J, Paton D
Department of Virology, Central Veterinary Laboratory (Weybridge), Addlestone, Surrey, UK.
J Gen Virol. 1996 Jun;77 ( Pt 6):1311-21. doi: 10.1099/0022-1317-77-6-1311.
By analysing the nucleotide sequence data generated from both the E2 (gp55) and the NS5B genes of classical swine fever virus (CSFV), in addition to previously published data from the 5'NCR, we were able to divide 115 CSFV isolates into two major groups, five subgroups and two disparate isolates. Further discrimination was possible by analysis of sequence data from the E2 region. The three sequencing based methods were compared to monoclonal antibody (MAb) typing and to limited restriction enzyme (RE) mapping. Although both MAb and RE methods confirmed the previous classification the resolution was inferior. We estimated an approximate evolution rate for CSFV from an analysis of the virus variation observed in a single geographical area over a 6 year period. Applying this proposed rate to each of our deduced CSFV subgroups enabled us to calculate the approximate dates of divergence for each subgroup.
通过分析经典猪瘟病毒（CSFV）E2（gp55）基因和NS5B基因产生的核苷酸序列数据，以及先前发表的来自5'NCR的数据，我们能够将115株CSFV分离株分为两个主要组、五个亚组和两个不同的分离株。通过分析E2区域的序列数据可以进行进一步区分。将三种基于测序的方法与单克隆抗体（MAb）分型和有限的限制性酶切图谱分析进行了比较。尽管MAb和RE方法都证实了先前的分类，但分辨率较低。通过分析在一个地理区域6年内观察到的病毒变异，我们估计了CSFV的大致进化速率。将这个提议的速率应用于我们推导的每个CSFV亚组，使我们能够计算每个亚组的大致分化日期。
