豌豆种传花叶病毒致病型P-1和致病型P-4分离株的生物学和分子特性
Biological and molecular properties of a pathotype P-1 and a pathotype P-4 isolate of pea seed-borne mosaic virus.
作者信息
Johansen I E, Keller K E, Dougherty W G, Hampton R O
机构信息
Department of Microbiology, Oregon State University, Corvallis 97331-3804, USA.
出版信息
J Gen Virol. 1996 Jun;77 ( Pt 6):1329-33. doi: 10.1099/0022-1317-77-6-1329.
Two isolates of pea seed-borne mosaic potyvirus, DPD1 and NY, were identified as pathotypes P-1 and P-4, respectively, by their infectivity on Pisum sativum L. lines homozygous for the recessive resistance genes sbm-1 and sbm-4. The two isolates differed in several biological characteristics. DPD1 induced transient vein clearing, downward rolling of leaflets and internode shortening on P. sativum, whereas NY only caused a slight growth reduction. DPD1 moved systemically in Chenopodium quinoa whereas NY was restricted to inoculated leaves. DPD1 was frequently transmitted by seeds whereas NY was rarely seed-transmitted: 24% and 0.3%, respectively, in P. sativum '549'. Both DPD1 and NY were transmitted by aphids (Myzus persicae), though a DAG triplet was not present in the N terminus of the coat protein. The nucleotide sequence and deduced amino acid sequence of NY were determined and compared to the corresponding sequences of DPD1.
通过对携带隐性抗性基因sbm - 1和sbm - 4的纯合豌豆品系的感染性鉴定，两种豌豆种传花叶马铃薯Y病毒分离株DPD1和NY分别被鉴定为致病型P - 1和P - 4。这两种分离株在几个生物学特性上存在差异。DPD1在豌豆上引起短暂的叶脉褪绿、小叶向下卷曲和节间缩短，而NY只导致轻微的生长减缓。DPD1能在藜麦中系统移动，而NY局限于接种叶片。DPD1经常通过种子传播，而NY很少通过种子传播：在豌豆‘549’中分别为24%和0.3%。DPD1和NY都能通过蚜虫（桃蚜）传播，尽管外壳蛋白N端不存在DAG三联体。测定了NY的核苷酸序列和推导的氨基酸序列，并与DPD1的相应序列进行了比较。
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