Johansen I E, Dougherty W G, Keller K E, Wang D, Hampton R O
Biotechnology Group, The Danish Institute of Plant and Soil Science, Lyngby, Denmark.
J Gen Virol. 1996 Dec;77 ( Pt 12):3149-54. doi: 10.1099/0022-1317-77-12-3149.
Two pea seedborne mosaic potyvirus (PSbMV) isolates, P-1 DPD1 (P-1), which is highly seed-transmitted, and P-4 NY (P-4), which is rarely seed-transmitted, and chimeras between P-1 and P-4 were analysed to map the viral genetic determinants of seed transmission. Infectivity of chimeric viruses was evaluated by inoculating Pisum sativum with RNA transcribed in vitro from recombinant full-length cDNA clones. The chimeric viruses that were used demonstrated that a genomic segment encoding the 49 kDa protease and putative RNA polymerase was responsible for symptom induction. Attempts to determine transmission of the chimeric viruses in P. sativum cultivars known to transmit P1 at high frequencies showed that seed transmission is a quantitative character influenced by multiple viral determinants. Seed transmission frequency did not correlate with accumulation of virus in vegetative tissue. The 5' 2.5 kb of the 10 kb PSbMV genome had a major influence on the seed transmission frequency and was analysed further. This showed that, while the helper-component protease was a major determinant of seed transmission, the potyviral P1 -protease exerted no measurable influence.
对两种豌豆种传花叶马铃薯Y病毒（PSbMV）分离株进行了分析，以确定种子传播的病毒遗传决定因素。这两种分离株分别是种子传播率高的P-1 DPD1（P-1）和种子传播率低的P-4 NY（P-4），以及P-1和P-4之间的嵌合体。通过用从重组全长cDNA克隆体外转录的RNA接种豌豆来评估嵌合病毒的感染性。所使用的嵌合病毒表明，编码49 kDa蛋白酶和假定RNA聚合酶的基因组片段负责症状诱导。在已知能高频传播P1的豌豆品种中，尝试确定嵌合病毒的传播情况，结果表明种子传播是一个受多种病毒决定因素影响的数量性状。种子传播频率与病毒在营养组织中的积累无关。对PSbMV 10 kb基因组的5' 2.5 kb区域进行了进一步分析，该区域对种子传播频率有主要影响。结果表明，虽然辅助成分蛋白酶是种子传播的主要决定因素，但马铃薯Y病毒P1蛋白酶没有可测量的影响。
