Zhou C, Shunji C, Jinsheng D, Junlin L, Jori G, Milanesi C
Laboratory of Electron Microscopy, Chinese Academy of Medical Sciences, Beijing, People's Republic of China.
J Photochem Photobiol B. 1996 May;33(3):219-23. doi: 10.1016/1011-1344(95)07250-0.
The destructive process of mouse MS-2 fibrosarcoma induced by photodynamic therapy (PDT) with liposome-administered Zn(II)-phthalocyanine (ZnPc) was studied by electron microscopy. Pronounced ultrastructural changes characteristic of apoptosis were observed for several tumour cells, including early occurrence of condensation and margination of chromatin, disappearance of nuclear pores, karyopyknosis, karyorrhexis, protuberance formation at the cell surface and cell fragmentation. The findings indicate that apoptosis was involved in the process of tumour cell death induced by ZnPc-PDT. The detailed mechanism and pathways controlling this phenomenon need to be elucidated further.
通过电子显微镜研究了用脂质体包裹的锌（II）-酞菁（ZnPc）进行光动力疗法（PDT）诱导的小鼠MS-2纤维肉瘤的破坏过程。在几个肿瘤细胞中观察到了明显的凋亡特征性超微结构变化，包括染色质早期出现凝聚和边缘化、核孔消失、核固缩、核碎裂、细胞表面形成突起和细胞碎片化。这些发现表明凋亡参与了ZnPc-PDT诱导的肿瘤细胞死亡过程。控制这一现象的详细机制和途径需要进一步阐明。
