Stiel H, Teuchner K, Paul A, Leupold D, Kochevar I E
Max-Born-Institut für Nichtlineare Optik und Kurzzeitspektroskopie, Berlin, Germany.
J Photochem Photobiol B. 1996 May;33(3):245-54. doi: 10.1016/1011-1344(95)07248-9.
A quantitative (multistep) excitation-deactivation model of rose bengal (RB) has been developed which includes energy and electron transfer to oxygen and the possibility of photoproduct formation via higher excited triplet-singlet states. The model is based on previous measurements of non-linear absorption (NLA) and emission of RB with picosecond pulses at 532 nm and on NLA measurements with nanosecond pulses. A coupled rate equation and photon transport equation approach for non-linear light-matter interaction is used. The resulting term scheme with all relevant excited state parameters confirms that (i) in the first excited state of RB, relevant absorption at 532 nm takes place only in the triplet, and (ii) the previously reported intensity dependence of RB-sensitized enzyme inhibition is well modelled by the intensity-dependent RB-T1 population and (as the main process) subsequent energy transfer to form singlet oxygen.
已经建立了孟加拉玫瑰红（RB）的定量（多步）激发-失活模型，该模型包括能量和电子向氧的转移以及通过更高激发的三重态-单重态形成光产物的可能性。该模型基于先前在532 nm处用皮秒脉冲对RB的非线性吸收（NLA）和发射的测量以及用纳秒脉冲进行的NLA测量。采用了用于非线性光-物质相互作用的耦合速率方程和光子输运方程方法。所得的具有所有相关激发态参数的能级图证实：（i）在RB的第一激发态中，532 nm处的相关吸收仅在三重态中发生，并且（ii）先前报道的RB敏化酶抑制的强度依赖性可以通过强度依赖性的RB-T1布居以及（作为主要过程）随后的能量转移以形成单线态氧来很好地模拟。
