Sutherland R S, Baskin L S, Hayward S W, Cunha G R
Department of Urology, University of California San Francisco, USA.
J Urol. 1996 Aug;156(2 Pt 2):571-7. doi: 10.1097/00005392-199608001-00002.
To study the cellular events occurring during bladder development and regeneration we developed an in vivo model of bladder augmentation with an acellular tissue graft. We propose that the extracellular matrix orchestrates the regenerative capacity of host bladder cells (urothelium, smooth muscle, blood vessels and nerve cells) after bladder augmentation with acellular tissue matrix.
A total of 40 adult rats underwent partial cystectomy and augmentation with a patch of extracellular matrix representing the full thickness of rat gastric or bladder tissue. Sections were examined histologically to assess urothelial, smooth muscle and neuronal invasion of the graft.
A total of 32 rats was evaluated 1 day to 26 weeks after grafting. Epithelialization occurred by day 4, accompanied by granulocytic infiltration. Smooth muscle regenerated 2 weeks after grafting in juxtaposition to epithelial surfaces and it matured into normal sized bundles by 26 weeks. Neovascularity was noted 2 weeks postoperatively. Neural elements formed around developing smooth muscle bundles as early as 4 weeks after grafting.
We demonstrated the regeneration of urothelium, smooth muscle, blood vessels and nerves within a full thickness grafted acellular tissue matrix scaffold in the rat. The spatial orientation of these elements suggests that mesenchymal-epithelial interactions occur during phenotypic regeneration of the bladder. Urothelium appears to regulate the early forming smooth muscle. This in vivo model provides a suitable method to study cellular events during regeneration.
为了研究膀胱发育和再生过程中发生的细胞事件，我们构建了一种使用无细胞组织移植物进行膀胱扩大术的体内模型。我们提出，细胞外基质在使用无细胞组织基质进行膀胱扩大术后，协调宿主膀胱细胞（尿路上皮、平滑肌、血管和神经细胞）的再生能力。
总共40只成年大鼠接受了部分膀胱切除术，并用一片代表大鼠胃或膀胱组织全层厚度的细胞外基质进行扩大术。对切片进行组织学检查，以评估移植物的尿路上皮、平滑肌和神经细胞浸润情况。
总共32只大鼠在移植后1天至26周接受评估。术后第4天发生上皮化，伴有粒细胞浸润。移植后2周，平滑肌在与上皮表面相邻处再生，并在26周时成熟为正常大小的束状结构。术后2周观察到新生血管形成。早在移植后4周，神经成分就在发育中的平滑肌束周围形成。
我们证明了大鼠全层移植的无细胞组织基质支架内尿路上皮、平滑肌、血管和神经的再生。这些成分的空间取向表明，在膀胱表型再生过程中发生了间充质-上皮相互作用。尿路上皮似乎调节早期形成的平滑肌。这种体内模型为研究再生过程中的细胞事件提供了一种合适的方法。
