Piovesana Tadeu Ravazi, Rodrigues Lenize da Silva, Bovolato Ana Livia de Carvalho, Rodríguez-Sánchez Diego Noé, Rinaldi Jaqueline Carvalho, Santos Nilton José, Mori Julia Calvi, Lourenção Pedro Luiz Toledo de Arruda, Birch Lynn, Bertanha Matheus
Department of Surgery and Orthopedics, Botucatu Medical School, São Paulo State University-UNESP, Botucatu 18618-687, Brazil.
Applied Biotechnology Laboratory, Clinical Hospital of Botucatu Medical School, São Paulo State University-UNESP, Botucatu 18618-687, Brazil.
Biomedicines. 2022 Nov 4;10(11):2814. doi: 10.3390/biomedicines10112814.
To evaluate tissue regeneration of the urinary bladder after the implantation of a decellularized vein sown with autologous adipose-derived mesenchymal stem cells (ASC) on luminal surfaces.
New Zealand rabbits ( = 10) were distributed in two groups: Group Bioscaffold alone (G1)-decellularized vena cava (1 cm) was implanted, and Group Bioscaffold plus ACSs (G2)-decellularized vena cava (1 cm) containing ASCs were implanted. ASCs were expanded, characterized, and maintained for one week in culture with a decellularized vein scaffold. The implants were performed under general anesthesia using a continuous suture pattern. Afterward, 21 d (day) specimens were collected and analyzed by hematoxylin and eosin (HE) histology and scanning electron microscopy (SEM).
The integrity of the urinary bladder was maintained in both groups. A superior regenerative process was observed in the G2 group, compared to the G1 group. We observed a greater urothelial epithelialization and maturity of the mucosa and submucosa fibroblasts. Furthermore, SEM demonstrated a notable amount of urothelial villus in the G2 group.
Decellularized vena cava scaffolds were able to maintain the integrity of the urinary bladder in the proposed model. In addition, ASCs accelerated the regenerative process development, observed primarily by the new urothelial epithelization and the maturity of mucosa and submucosa fibroblasts.
评估在腔面播种自体脂肪间充质干细胞（ASC）的去细胞静脉植入后膀胱的组织再生情况。
将10只新西兰兔分为两组：单独生物支架组（G1）——植入1厘米去细胞腔静脉；生物支架加ASC组（G2）——植入含有ASC的1厘米去细胞腔静脉。ASC经扩增、鉴定后，与去细胞静脉支架一起在培养中维持一周。植入手术在全身麻醉下采用连续缝合方式进行。之后，收集21天的标本，通过苏木精和伊红（HE）组织学及扫描电子显微镜（SEM）进行分析。
两组膀胱的完整性均得以维持。与G1组相比，G2组观察到更好的再生过程。我们观察到G2组有更大量的尿路上皮化生以及黏膜和黏膜下成纤维细胞的成熟。此外，SEM显示G2组有大量的尿路上皮绒毛。
在所提出的模型中，去细胞腔静脉支架能够维持膀胱的完整性。此外，ASC加速了再生过程的发展，主要表现为新的尿路上皮化生以及黏膜和黏膜下成纤维细胞的成熟。
