Hussain R, Chaudhry N A, Anwar M S, Khan S A, Munir M, Tayyab M
Department of Pathology, Postgraduate Medical Institute, Lahore.
J Pak Med Assoc. 1996 Feb;46(2):38-41.
Two hundred and fifty cases of clinically suspected urinary tract infection were analysed for the detection of bacteriuria. Parameters studied included direct Gram staining, pyuria on microscopic examination of uncentrifuged urine and dip strip method for the detection of blood, protein, nitrite and leucocyte esterase. Significant bacteriuria (colony count 10(5) per ml) was found in 112 cases with a positivity ranging from 65 to 83% for the presence of blood, protein, nitrite and leucocyte esterase. Highest positive predictive values were obtained with the presence of nitrite and leucocyte esterase (98%), blood, protein and nitrite (94%) as well as with blood, protein, nitrite and leucocyte esterase (98%). Both pyuria and direct Gram staining were positive in 85% cases. The combined presence of both these parameters gave 100% positive predictive value. Gram staining combined with pyuria was more effective and economical as compared to the dipstrips for the detection of bacteriuria.
对250例临床疑似尿路感染的病例进行了菌尿检测分析。研究的参数包括直接革兰氏染色、未离心尿液显微镜检查中的脓尿以及检测血液、蛋白质、亚硝酸盐和白细胞酯酶的试纸条法。在112例病例中发现显著菌尿(菌落计数每毫升10⁵),血液、蛋白质、亚硝酸盐和白细胞酯酶存在时的阳性率为65%至83%。亚硝酸盐和白细胞酯酶存在时(98%)、血液、蛋白质和亚硝酸盐存在时(94%)以及血液、蛋白质、亚硝酸盐和白细胞酯酶存在时(98%)获得最高的阳性预测值。85%的病例脓尿和直接革兰氏染色均为阳性。这两个参数同时存在时阳性预测值为100%。与试纸条法相比,革兰氏染色结合脓尿在检测菌尿方面更有效且更经济。