Espinosa-Aguirre J J, Rubio J, Cassani M, Nosti R, Caballero S, González I, Martínez G
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, México, D.F.
Mutat Res. 1996 Jun 12;368(2):103-7. doi: 10.1016/0165-1218(95)00083-6.
The S9 fraction obtained from rats orally pretreated for 3 days with cyclohexanol was able to activate the pro-mutagen N-nitrosodimethylamine (NDMA) into highly mutagenic metabolite(s) detected in the TA100 strain of Salmonella typhimurium. NDMA was not mutagenic when uninduced S9 was used as metabolic source but was approximately twice more mutagenic with cyclohexanol-induced S9 compared to ethanol-induced S9. Separation of microsomal proteins by sodium dodecylsulfate gel electrophoresis, displayed protein bands situated in the range of 50,000 to 52,000 molecular weight induced by both, ethanol and cyclohexanol. These results are evidence of the induction properties of cyclohexanol.
用环己醇对大鼠进行3天口服预处理后获得的S9组分，能够将前诱变剂N-亚硝基二甲胺（NDMA）激活为在鼠伤寒沙门氏菌TA100菌株中检测到的高诱变性代谢物。当使用未诱导的S9作为代谢源时，NDMA没有诱变性，但与乙醇诱导的S9相比，环己醇诱导的S9使NDMA的诱变性大约高两倍。通过十二烷基硫酸钠凝胶电泳分离微粒体蛋白，显示乙醇和环己醇诱导的蛋白条带位于分子量50,000至52,000范围内。这些结果证明了环己醇的诱导特性。
