Bankole S A, Eseigbe D A, Enikuomehin O A
Department of Biological Sciences, Ogun State University, Ago-Iwoye, Nigeria.
Mycopathologia. 1995;132(3):155-60. doi: 10.1007/BF01103981.
The mycoflora, moisture content and aflatoxin contamination of pigeon pea (Cajanus cajan (L.) Millisp) stored in jute sacks and iron bins were determined at monthly intervals for a year. The predominant fungi on freshly harvested seeds were Alternaria spp., Botryodiplodia theobromae, Fusarium spp. and Phoma spp. These fungi gradually disappeared from stored seeds with time and by 5-6 months, most were not isolated. The fungi that succeeded the initially dominant ones were mainly members of the general Aspergillus, Penicillium and Rhizopus. Population of these fungi increased up to the end of one year storage. Higher incidence of mycoflora and Aspergillus flavus were recorded in jute-sack samples throughout the storage period. The moisture content of stored seeds was found to fluctuate with the prevailing weather conditions, being low during the dry season and slightly high during the wet season. The stored seeds were free of aflatoxins for 3 and 5 months in jute sacks and iron bins respectively. The level of aflatoxins detected in jute-sack storage system was considerably higher than that occurring in the iron bin system. Of 196 isolates of A. flavus screened, 48% were toxigenic in liquid culture (54% from jute sacks and 41% from iron bins).
对储存在黄麻袋和铁桶中的木豆（Cajanus cajan (L.) Millisp）的霉菌菌群、水分含量和黄曲霉毒素污染情况进行了为期一年的每月一次的测定。刚收获的种子上的主要真菌有链格孢属、可可球二孢、镰刀菌属和茎点霉属。随着时间的推移，这些真菌在储存的种子中逐渐消失，到5 - 6个月时，大多数已无法分离得到。取代最初占主导地位真菌的真菌主要是曲霉属、青霉属和根霉属的成员。这些真菌的数量在储存一年结束时有所增加。在整个储存期间，黄麻袋样品中的霉菌菌群和黄曲霉的发生率更高。发现储存种子的水分含量随当时的天气条件而波动，旱季时较低，雨季时略高。储存的种子在黄麻袋和铁桶中分别有3个月和5个月未检测到黄曲霉毒素。在黄麻袋储存系统中检测到的黄曲霉毒素水平明显高于铁桶系统。在筛选的196株黄曲霉分离株中，48%在液体培养中具有产毒能力（黄麻袋中的为54%，铁桶中的为41%）。
