Reeves S A, Ueki K, Sinha B, Difiglia M, Louis D N
Molecular Neuro-Oncology Laboratory, Massachusetts General Hospital, Charlestown, USA.
Neuroscience. 1996 Apr;71(4):1037-42. doi: 10.1016/0306-4522(95)00491-2.
The protein tyrosine phosphatase SH-PTP2 has been implicated in a variety of cell signaling cascades, including those mediating neuronal survival. We therefore investigated the expression of SH-PTP2 in the adult human nervous system using Western blotting, immunohistochemistry and immunoelectron microscopy. SH-PTP2 immunoreactivity was noted only in neurons, but was not restricted to a specific neuronal type or location. Immunohistochemistry showed perikaryal staining, whereas Western blotting and ultrastructural analysis suggested that SH-PTP2 is present in axons as well. While immunohistochemistry showed a Nissl-like pattern in large motor neurons, immunoelectron microscopy demonstrated a diffuse pattern of cytoplasmic staining, without apparent preferential localization. The presence of the SH2 domain-containing tyrosine-specific phosphatase SH-PTP2 in diverse neurons in the adult nervous system suggests that SH-PTP2 may play a role in a broad spectrum of neuronal responses.
蛋白酪氨酸磷酸酶SH-PTP2参与了多种细胞信号转导通路，包括介导神经元存活的通路。因此，我们采用蛋白质免疫印迹法、免疫组织化学法和免疫电子显微镜法，研究了SH-PTP2在成人人神经系统中的表达情况。结果发现，SH-PTP2免疫反应性仅存在于神经元中，但并不局限于特定的神经元类型或位置。免疫组织化学显示核周染色，而蛋白质免疫印迹法和超微结构分析表明，轴突中也存在SH-PTP2。虽然免疫组织化学在大型运动神经元中显示出类似尼氏体的模式，但免疫电子显微镜显示胞质染色呈弥漫性模式，无明显的优先定位。成年神经系统中不同神经元中含有SH2结构域的酪氨酸特异性磷酸酶SH-PTP2的存在表明，SH-PTP2可能在广泛的神经元反应中发挥作用。
