Sedbrook J C, Kronebusch P J, Borisy G G, Trewavas A J, Masson P H
Laboratory of Genetics, University of Wisconsin-Madison 53706, USA.
Plant Physiol. 1996 May;111(1):243-57. doi: 10.1104/pp.111.1.243.
Using the transgenic AEQUORIN system, we showed that the cotyledons and leaves of Arabidopsis thaliana seedlings developed a biphasic luminescence response to anoxia, indicating changes in cytosolic Ca2+ levels. A fast and transient luminescence peak occurred within minutes of anoxia, followed by a second, prolonged luminescence response that lasted 1.5 to 4 h. The Ca2+ channel blockers Gd3+, La3+, and ruthenium red (RR) partially inhibited the first response and promoted a larger and earlier second response, suggesting different origins for these responses. Both Gd3+ and RR also partially inhibited anaerobic induction of alcohol dehydrogenase gene expression. However, although anaerobic alcohol dehydrogenase gene induction occurred in seedlings exposed to water-agar medium and in roots, related luminescence responses were absent. Upon return to normoxia, the luminescence of cotyledons, leaves, and roots dropped quickly, before increasing again in a Gd3+, La3+, ethyleneglycol-bis(beta-aminoethyl ether)-N,N'-tetraacetic acid-, and RR-sensitive fashion.
利用转基因水母发光蛋白系统，我们发现拟南芥幼苗的子叶和叶片对缺氧产生了双相发光反应，这表明胞质Ca2+水平发生了变化。缺氧后几分钟内出现快速且短暂的发光峰值，随后是持续1.5至4小时的第二次延长的发光反应。Ca2+通道阻滞剂Gd3+、La3+和钌红（RR）部分抑制了第一次反应，并促进了更大且更早的第二次反应，表明这些反应的起源不同。Gd3+和RR也部分抑制了酒精脱氢酶基因表达的厌氧诱导。然而，尽管在暴露于水琼脂培养基的幼苗和根中发生了厌氧酒精脱氢酶基因诱导，但未观察到相关的发光反应。恢复到常氧状态后，子叶、叶片和根的发光迅速下降，然后以对Gd3+、La3+、乙二醇双（β-氨基乙基醚）-N,N'-四乙酸和RR敏感的方式再次增加。
