Deriabin P G, Loginova N V
Vopr Virusol. 1995 Nov-Dec;40(6):265-8.
Attenuated strain of Japanese encephalitis virus (JEV), clone 3, has been isolated from suckling mouse brain cell cultures chronically infected with JEV Nakayama strain, clone 33. Clone 3 actively replicates in 9-day chicken fibroblast cultures and possessed an uncommon for a JE agent capacity of causing cytodestruction of 1-2-day monolayer of chicken embryo cells, accumulates in these cultures in high titers (9.0 lg PFU/ml and higher), and is characterized by a high antigenic activity and a wide spectrum of antigenic relationships. New biologic properties acquired by JEV over the course of its persistence were retained after 10 and more passages through suckling mouse brain.
从长期感染日本脑炎病毒（JEV）中山株33克隆的乳鼠脑细胞培养物中分离出了JEV减毒株3克隆。3克隆在9日龄鸡成纤维细胞培养物中能活跃复制，具有一种对JE病原体来说不常见的能力，即能导致1 - 2日龄鸡胚细胞单层发生细胞破坏，在这些培养物中高滴度积累（9.0 lg PFU/ml及更高），并且具有高抗原活性和广泛的抗原关系谱。JEV在其持续存在过程中获得的新生物学特性在通过乳鼠脑传代10次及更多次后仍得以保留。
