Oreshkova S F, Glotov A G, Semenikhin V I, Minenkova O O, Puzyrev A T, Tikunova N V, Baturina I I, Petrenko V A, Il'ichev A A
Vopr Virusol. 1995 Nov-Dec;40(6):279-82.
Biotin-labeled DNA probes for infectious bovine rhinotracheitis virus also known as bovine herpesvirus-1 (BHV-1) have been developed. The procedure is based on dot-blot hybridization using biotin-labeled bacteriophage M13 and plasmid probes containing cloned PstI and EcoRI-PstI restriction fragments of viral genome. The probes obtained were used to detect viral nucleic acids in specimens of bovine spermatic fluid or nasal swabs of calves. The method is simple and rapid, taking less than 24 h, and is highly specific and sensitive, this recommending it for practical veterinary.
已开发出用于检测传染性牛鼻气管炎病毒（也称为牛疱疹病毒1型，即BHV - 1）的生物素标记DNA探针。该方法基于斑点杂交，使用生物素标记的噬菌体M13和含有病毒基因组克隆的PstI和EcoRI - PstI限制性片段的质粒探针。所获得的探针用于检测牛精液或犊牛鼻拭子样本中的病毒核酸。该方法简单快速，耗时不到24小时，且具有高度特异性和敏感性，因此推荐用于实际兽医工作中。
