采用斑点杂交法检测牛疱疹病毒1型核酸序列。
Detection of bovine herpesvirus-1 nucleic acid sequences, using a dot-blot hybridization procedure.
作者信息
Andino R H, Torres H N, Polacino P S, Schudel A, Palma E L
出版信息
Am J Vet Res. 1987 Jun;48(6):984-7.
PMID:3037953
Abstract
A sensitive and specific procedure for the detection of Argentine isolates of bovine herpesvirus-1 was developed. The procedure was based on a dot-blot, nucleic acid hybridization, using 32P, nick-translated, plasmidic probes. The probes contained cloned Bam H1 restriction fragments in the left half of the viral genome. The detection limit of the procedure was 10 pg of viral DNA.
摘要
开发了一种用于检测牛疱疹病毒-1阿根廷分离株的灵敏且特异的方法。该方法基于斑点印迹核酸杂交，使用经缺口平移用32P标记的质粒探针。这些探针包含病毒基因组左半部分克隆的Bam H1限制性片段。该方法的检测限为10 pg病毒DNA。
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