原弹性蛋白的蛋白水解作用。
Proteolysis of tropoelastin.
作者信息
Mecham R, Foster J A, Franzblau C
出版信息
Adv Exp Med Biol. 1977;79:209-20. doi: 10.1007/978-1-4684-9093-0_18.
PMID:868636
Abstract
The presence of an enzyme associated with tropoelastin is described. The enzyme has a pH optimum between 7 and 9 and trypsin-like specificity. Upon incubation, tropoelastin (72,000 molecular weight) is cleaved into relatively high molecular weight fragments. In addition to the parent molecule, five discrete polypeptide bands are usually observed on SDS gels with molecular weights of approximately 57,000, 45,000, 36,000, 25,000 and 13-14,000.
摘要
描述了一种与原弹性蛋白相关的酶。该酶的最适pH值在7至9之间，具有类胰蛋白酶特异性。孵育后，原弹性蛋白（分子量72,000）被切割成相对高分子量的片段。除了亲本分子外，在SDS凝胶上通常观察到五条离散的多肽带，分子量约为57,000、45,000、36,000、25,000和13 - 14,000。
相似文献
1
Proteolysis of tropoelastin.原弹性蛋白的蛋白水解作用。
Adv Exp Med Biol. 1977;79:209-20. doi: 10.1007/978-1-4684-9093-0_18.
2
The anti-proteolytic behavior of lathyrogens.豆科中毒素的抗蛋白水解作用。
Biochim Biophys Acta. 1979 Nov 1;587(4):477-83. doi: 10.1016/0304-4165(79)90001-1.
3
Trypsin-like neutral protease associated with soluble elastin.与可溶性弹性蛋白相关的胰蛋白酶样中性蛋白酶。
Biochemistry. 1977 Aug 23;16(17):3825-31. doi: 10.1021/bi00636a017.
4
Effect of protease inhibitors and substrates on deoxycorticosterone binding to its receptor in dog MDCK kidney cells.蛋白酶抑制剂和底物对脱氧皮质酮与犬MDCK肾细胞中其受体结合的影响。
Nature. 1977 Oct 27;269(5631):810-2. doi: 10.1038/269810a0.
5
Studies on the elastolytic activity of chymotrypsin.关于胰凝乳蛋白酶弹性溶解活性的研究。
Connect Tissue Res. 1985;13(4):291-8. doi: 10.3109/03008208509152410.
6
Inhibition of elastolysis by proteinase inhibitors from chick plasma and aorta.鸡血浆和主动脉中蛋白酶抑制剂对弹性蛋白溶解的抑制作用。
Biochim Biophys Acta. 1978 Mar 20;539(3):267-75. doi: 10.1016/0304-4165(78)90031-4.
7
Effects of protease inhibitors on early stages of meiosis in Xenopus laevis oocytes.蛋白酶抑制剂对非洲爪蟾卵母细胞减数分裂早期阶段的影响。
Exp Cell Res. 1975 Mar 15;91(2):460-4.
8
Resistance of tropoelastin and elastin peptides to degradation by alpha 2-macroglobulin-protease complexes.原弹性蛋白和弹性蛋白肽对α2-巨球蛋白-蛋白酶复合物降解的抗性。
Arch Biochem Biophys. 1981 Oct 1;211(1):143-50. doi: 10.1016/0003-9861(81)90439-2.
9
10
Schistosoma mansoni: purification and characterization of the major acidic proteinase from adult worms.曼氏血吸虫：成虫主要酸性蛋白酶的纯化与特性分析
Exp Parasitol. 1986 Jun;61(3):398-404. doi: 10.1016/0014-4894(86)90196-7.
Zotero 插件