气管内注入的14C-胍基化胰弹性蛋白酶在仓鼠肺中的命运。

The fate of intratracheal 14C-guanidinated pancreatic elastase in hamster lung.

作者信息

Stone P J, Pereira V, Biles D, Snider G L, Kagan H M, Franzblau C

出版信息

Adv Exp Med Biol. 1977;79:233-48. doi: 10.1007/978-1-4684-9093-0_20.

Abstract

Native pancreatic elastase and guanidinated elastase have similar in vitro and in vivo properties and produce emphysema of similar severity in hamsters. 14C-guanidinated pancreatic elastase (16,000 cpm/0.2 mg) was instilled into the trachea of anesthetized hamsters. Within 24 hours the radiolabel found in the lungs rapidly decreases to 12% of the original 16,000 cpm and to 1% after 96 hours. Most of the radiolabel and elastase activity found in the lungs can be removed by bronchopulmonary lavage up to 48 hours after installation. Although seemingly very small, there is a significant amount of radiolabel (1-2%) which cannot be removed from the lungs by extensive bronchopulmonary lavage.

摘要

天然胰腺弹性蛋白酶和胍基化弹性蛋白酶具有相似的体外和体内特性，并且在仓鼠中产生严重程度相似的肺气肿。将14C-胍基化胰腺弹性蛋白酶（16,000计数/分钟/0.2毫克）注入麻醉仓鼠的气管。在24小时内，肺中发现的放射性标记迅速降至原始16,000计数/分钟的12%，96小时后降至1%。在注入后长达48小时，通过支气管肺灌洗可以清除肺中发现的大部分放射性标记和弹性蛋白酶活性。尽管看起来非常少，但仍有大量放射性标记（1%-2%）无法通过广泛的支气管肺灌洗从肺中清除。