对培养的人皮肤成纤维细胞释放到培养基中的主要岩藻糖基化糖蛋白的研究。

Studies on the major fucosylated glycoprotein released into the medium by cultured human skin fibroblasts.

作者信息

Sear C H, Grant M E, Jackson D S

出版信息

Adv Exp Med Biol. 1977;79:375-84. doi: 10.1007/978-1-4684-9093-0_33.

Abstract

Electrophoretic analysis of the proteins synthesized by cultured human skin fibroblasts demonstrated that the major component released into the medium is a high molecular weight fucosylated glycoprotein (MFGP). Gel filtration chromatography under denaturing and reducing conditions indicated that MFGP has a molecular weight of approx. 250,000; but appears to behave as a disulphide-linked dimer when unreduced. MFGP is further distinguished as the major labelled macromolecule in the medium after incubation of fibroblasts with [35S]cysteine. The role of this glycoprotein is unknown but it bears a striking resemblance to a presumptive structural glycoprotein recently shown to be secreted by arterial smooth muscle cells in culture, and may also be related to a group of much studied cell surface glycoproteins.

摘要

对培养的人皮肤成纤维细胞合成的蛋白质进行电泳分析表明，释放到培养基中的主要成分是一种高分子量岩藻糖基化糖蛋白（MFGP）。在变性和还原条件下进行的凝胶过滤色谱分析表明，MFGP的分子量约为250,000；但在未还原时似乎表现为二硫键连接的二聚体。在用[35S]半胱氨酸孵育成纤维细胞后，MFGP是培养基中主要的标记大分子。这种糖蛋白的作用尚不清楚，但它与最近在培养中显示由动脉平滑肌细胞分泌的一种假定的结构糖蛋白有显著相似之处，并且可能也与一组研究较多的细胞表面糖蛋白有关。