Ji X, Kondo K, Aramaki Y, Kricka L J
Department of Pathology and Laboratory Medicine, University of Pennsylvania, Philadelphia 19104, USA.
J Biolumin Chemilumin. 1996 Jan-Feb;11(1):1-7. doi: 10.1002/(SICI)1099-1271(199601)11:1<1::AID-BIO389>3.0.CO;2-2.
4-Substituted phenyl boronic acids (e.g., 4-iodo, 4-bromo, 4-phenyl) are effective enhancers of the horseradish peroxidase (Type VIA) catalysed chemiluminescent oxidation of various pyrido[3,4-d]pyridazine-1,4(2H,3H)dione derivatives. The most effective combination was 4-biphenylboronic acid and 8-amino-5-chloro-7-phenylpyrido[3,4-d]- pyridazine-1,4(2H,3H)dione. Generally, the intensity of light emission in the presence of peroxidase was higher with the pyridopyridazines than with sodium luminol. However, the blank light emission was much lower with sodium luminol than with the pyridopyridazines. A synergistic enhancement phenomenon was demonstrated for the combination of a 4-iodophenol and a 4-biphenylboronic acid enhancer with 8-amino-5-chloro-7-phenylpyrido[3,4-d]pyridazine-1,4(2H,3H) dione. The combination of these two enhancers produced a light emission intensity in an assay for 5 fmol of peroxidase that was 25% higher than expected from the sum of the individual light intensities.
4-取代苯基硼酸（例如，4-碘苯基、4-溴苯基、4-苯基）是辣根过氧化物酶（VIA型）催化的各种吡啶并[3,4-d]哒嗪-1,4(2H,3H)二酮衍生物化学发光氧化反应的有效增强剂。最有效的组合是4-联苯硼酸与8-氨基-5-氯-7-苯基吡啶并[3,4-d]哒嗪-1,4(2H,3H)二酮。一般来说，在过氧化物酶存在的情况下，吡啶并哒嗪类化合物的发光强度比鲁米诺钠更高。然而，鲁米诺钠的空白发光比吡啶并哒嗪类化合物低得多。对于4-碘苯酚和4-联苯硼酸增强剂与8-氨基-5-氯-7-苯基吡啶并[3,4-d]哒嗪-1,4(2H,3H)二酮的组合，表现出协同增强现象。在检测5飞摩尔过氧化物酶时，这两种增强剂的组合产生的发光强度比根据各自发光强度之和预期的高出25%。
