Okada Y, Copeland B R, Hamann G F, Koziol J A, Cheresh D A, del Zoppo G J
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, California, USA.
Am J Pathol. 1996 Jul;149(1):37-44.
The endothelial and smooth muscle integrin alphaVbeta3, a receptor for vitronectin and fibrinogen, participates in angiogenesis associated with wound healing and tumorigenicity. The microvascular expression of alphavbeta3 and fibrin during experimental middle cerebral artery occlusion and reperfusion in a nonhuman primate model was examined by computer-assisted video imaging microscopy. No microvascular expression of alphavbeta3 was seen in the control subjects (n = 3) or the non-ischemic basal ganglia of subjects undergoing 2-hour MCA:O (middle cerebral artery occlusion) or 3-hour occlusion with 1-hour (n = 3), 4-hour (n = 3), and 24-hour (n = 3) reperfusion. In the ischemic territory, alphavbeta3 appeared initially at 2 hours of middle cerebral artery occlusion. Up-regulation of alphavbeta3 was confined to the media of 30.0- to 50.0-micron-diameter arterioles in the ischemic core and correlated significantly with fibrin deposition in those vessels (P < 0.0005). Integrin alphavbeta3 and its ligand fibrinogen appear in a subpopulation of microvessels after focal cerebral ischemia.
内皮和平滑肌整合素αVβ3是玻连蛋白和纤维蛋白原的受体，参与与伤口愈合和肿瘤发生相关的血管生成。通过计算机辅助视频成像显微镜检查了非人类灵长类动物模型在实验性大脑中动脉闭塞和再灌注期间αVβ3和纤维蛋白的微血管表达。在对照组（n = 3）或接受2小时大脑中动脉闭塞（MCA:O）或3小时闭塞并分别再灌注1小时（n = 3）、4小时（n = 3）和24小时（n = 3）的受试者的非缺血性基底神经节中未观察到αVβ3的微血管表达。在缺血区域，αVβ3最初在大脑中动脉闭塞2小时时出现。αVβ3的上调局限于缺血核心区直径为30.0至50.0微米的小动脉中膜，并且与这些血管中的纤维蛋白沉积显著相关（P < 0.0005）。整合素αVβ3及其配体纤维蛋白原在局灶性脑缺血后的微血管亚群中出现。
