Kunishi M, Kayada Y, Tsumura M, Yoshiga K, Takada K
Dept. of Oral and Maxillofacial Surgery 1, Hiroshima University School of Dentistry, Japan.
Gan To Kagaku Ryoho. 1996 Jul;23(8):1045-8.
Using antihuman CD44 variant 6 monoclonal antibody (2F10), immunohistochemical screenings were performed for 38 oral squamous cell carcinoma cases and 10 oral mucosa in healthy cases as normal counterpart. Normal epithelium in the oral surface was stained intensely by the antibody. The reactivity was particularly strong in the spinous layers of stratified squamous epithelium. Cells in the basal layers exhibited moderate staining. In contrast, expression of CD44v6 tended to be downregulated in 38 oral squamous cell carcinoma materials. Interestingly, more faint or no staining by the anti-CD44v6 monoclonal antibody was found in the primary squamous cell carcinomas involving regional lymphnode metastasis. Downregulation of CD44v6 isoform was suggested to occur during regional lymphnode metastasis on oral squamous, cell carcinomas.
使用抗人CD44变异体6单克隆抗体（2F10），对38例口腔鳞状细胞癌病例和10例作为正常对照的健康口腔黏膜病例进行免疫组织化学筛查。口腔表面的正常上皮被该抗体强烈染色。在复层鳞状上皮的棘层中反应性尤其强烈。基底层的细胞呈现中度染色。相比之下，在38份口腔鳞状细胞癌材料中，CD44v6的表达倾向于下调。有趣的是，在伴有区域淋巴结转移的原发性鳞状细胞癌中发现抗CD44v6单克隆抗体的染色更淡或无染色。提示CD44v6异构体的下调发生在口腔鳞状细胞癌区域淋巴结转移过程中。
