胰岛素分泌细胞BRIN-BD11对己糖的识别。

Hexose recognition by insulin-secreting BRIN-BD11 cells.

作者信息

McClenaghan N H, Gray A M, Barnett C R, Flatt P R

机构信息

School of Biomedical Sciences, University of Ulster, Coleraine, United Kingdom.

出版信息

Biochem Biophys Res Commun. 1996 Jun 25;223(3):724-8. doi: 10.1006/bbrc.1996.0963.

DOI:10.1006/bbrc.1996.0963

PMID:8687464

Abstract

Clonal BRIN-BD11 cells were produced by electrofusion of NEDH rat islet B-cells with immortal RINm5F cells. Western blotting analysis revealed that unlike RINm5F, novel BRIN-BD11 cells expressed high levels of the glucose transport protein GLUT-2, coupled with a rapid and sustained uptake of D-glucose, significantly greater than RINm5F after only 5 min (p < 0.05). Whereas BRIN-BD11 cells expressed a high glucokinase:hexokinase ratio with 1.4-2.0 fold and 1.4-1.7 fold stepwise stimulation of insulin secretion with 4.2-16.7 mM D-glucose and D-mannose respectively, RINm5F had a lower glucokinase:hexokinase ratio (p < 0.001) and were notably unresponsive to D-glucose and D-mannose. Unlike RINm5F cells, BRIN-BD11 were unresponsive to other hexoses, with RINm5F only responding to D-galactose (p < 0.05). BRIN-BD11 cells should be useful for studies of nutrient-induced insulin secretion.

摘要

通过将NEDH大鼠胰岛B细胞与永生的RINm5F细胞进行电融合，产生了克隆性BRIN - BD11细胞。蛋白质印迹分析显示，与RINm5F不同，新型BRIN - BD11细胞表达高水平的葡萄糖转运蛋白GLUT - 2，伴随着D - 葡萄糖的快速持续摄取，仅在5分钟后就显著高于RINm5F（p < 0.05）。虽然BRIN - BD11细胞的葡萄糖激酶：己糖激酶比值较高，分别用4.2 - 16.7 mM D - 葡萄糖和D - 甘露糖对胰岛素分泌进行1.4 - 2.0倍和1.4 - 1.7倍的逐步刺激，但RINm5F的葡萄糖激酶：己糖激酶比值较低（p < 0.001），并且对D - 葡萄糖和D - 甘露糖明显无反应。与RINm5F细胞不同，BRIN - BD11对其他己糖无反应，而RINm5F仅对D - 半乳糖有反应（p < 0.05）。BRIN - BD11细胞应有助于营养诱导的胰岛素分泌研究。