Rasschaert J, Flatt P R, Barnett C R, McClenaghan N H, Malaisse W J
Laboratory of Experimental Medicine, Brussels Free University, Belgium.
Biochem Mol Med. 1996 Apr;57(2):97-105. doi: 10.1006/bmme.1996.0015.
A novel insulin-secreting cell line, BRIN-BD11, was recently established following electrofusion of RINm5F cells with NEDH rat pancreatic islet cells. In the present study, D-glucose metabolism was compared in BRIN-BD11 and RINm5F cells. The concentration dependency of D[5-3H]glucose utilization displayed a comparable pattern in the two cell lines, but the absolute values were lower in BRIN-BD11 than RINm5F cells. Except in the case of D-[1-14C]glucose, the ratio between 14C labeled D-glucose oxidation and D-[5-3H]glucose utilization was higher, however, in BRIN-BD11 than RINm5F cells. Moreover, BRIN-BD11 cells were less affected than RINm5F cells by a rise in D-glucose concentration, in terms of the inhibitory action of the hexose upon oxidative variables, such as oxidative glycolysis, pyruvate decarboxylation, and oxidation of glucose-derived acetyl residues in the Krebs cycle. The total energy yield from D-glucose catabolism appeared similar, however, in BRIN-BD11 and RINm5F cells. These findings extend the knowledge that BRIN-BD11 cells display an improved metabolic and secretory behavior, when considering the difference otherwise found between normal and tumoral islet cells.
一种新的胰岛素分泌细胞系BRIN-BD11，是最近通过将RINm5F细胞与NEDH大鼠胰岛细胞电融合而建立的。在本研究中，对BRIN-BD11细胞和RINm5F细胞中的D-葡萄糖代谢进行了比较。D-[5-³H]葡萄糖利用的浓度依赖性在两种细胞系中表现出相似的模式，但BRIN-BD11细胞中的绝对值低于RINm5F细胞。然而，除了D-[1-¹⁴C]葡萄糖的情况外，¹⁴C标记的D-葡萄糖氧化与D-[5-³H]葡萄糖利用之间的比率在BRIN-BD11细胞中高于RINm5F细胞。此外，就己糖对氧化变量（如氧化糖酵解、丙酮酸脱羧以及三羧酸循环中葡萄糖衍生的乙酰残基的氧化）的抑制作用而言，BRIN-BD11细胞受D-葡萄糖浓度升高的影响小于RINm5F细胞。然而，BRIN-BD11细胞和RINm5F细胞中D-葡萄糖分解代谢产生的总能量似乎相似。考虑到正常胰岛细胞和肿瘤胰岛细胞之间的其他差异，这些发现扩展了我们对BRIN-BD11细胞具有改善的代谢和分泌行为的认识。
