一种创建双链DNA生物传感器的新方法。
A new approach for creating double-stranded DNA biosensors.
作者信息
Skuridin S G, Yevdokimov Y M, Efimov V S, Hall J M, Turner A P
机构信息
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
出版信息
Biosens Bioelectron. 1996;11(9):903-11. doi: 10.1016/0956-5663(96)89439-5.
The principle of 'sandwich'-type biosensors based on liquid-crystalline dispersions formed from [DNA-polycation] complexes is outlined. These biosensors will find application in the determination of a range of compounds and physical factors that affect the ability of a given polycationic molecule to maintain intermolecular crosslinks between neighbouring DNA molecules. In the case of liquid-crystalline dispersions formed from [DNA-protamine] complexes, the lowest concentration of hydrolytic enzyme (trypsin) detectable was approximately 10-(14) M.
概述了基于由[DNA-聚阳离子]复合物形成的液晶分散体的“三明治”型生物传感器的原理。这些生物传感器将应用于测定一系列化合物和物理因素，这些因素会影响给定聚阳离子分子维持相邻DNA分子间分子间交联的能力。对于由[DNA-鱼精蛋白]复合物形成的液晶分散体，可检测到的水解酶（胰蛋白酶）的最低浓度约为10^(-14) M。
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