Kanwar S S, Tewari H K, Chadha B S, Punj V, Sharma V K
Department of Microbiology, College of Basic Sciences and Humanities, Punjab Agricultural University, Ludhiana, India.
Acta Microbiol Immunol Hung. 1995;42(4):331-8.
Sporolactobacillus cellulosolvens (NCIMB 12173) isolated from an anaerobic digester and characterised biochemically is being reported for homofermentative lactic acid production from molasses in a batch culture. The effect of various process parameters on lactic acid production were optimized. A maximum lactic acid (24.2 g/l) and yield coefficient (0.79) was achieved using 3% (v/v) inoculum of 36 h old culture in molasses medium containing sugars (5%; w/v) supplemented with peptone (2.5 g/l) and (NH4)2SO4 (7.5 g/l), pH 6.5 at 40 degrees C after 72 h of fermentation.
从厌氧消化器中分离出的并经生化特性鉴定的解纤维素芽孢乳杆菌(NCIMB 12173),现报道其在分批培养中由糖蜜同型发酵生产乳酸的情况。对各种工艺参数对乳酸生产的影响进行了优化。在含有5%(w/v)糖类、补充有2.5 g/l蛋白胨和7.5 g/l硫酸铵、pH值为6.5的糖蜜培养基中,使用36小时龄培养物的3%(v/v)接种量,在40℃下发酵72小时后,获得了最大乳酸产量(24.2 g/l)和产率系数(0.79)。