Hoffmann T F, Leiderer R, Waldner H, Messmer K
Institute for Surgical Research, Ludwig-Maximilians-Universität, Munich, Germany.
Br J Surg. 1996 Feb;83(2):189-95.
Ischaemia-reperfusion of the pancreas was performed in 35 anaesthetized Sprague-Dawley rats. The effects of two bradykinin antagonists, HOE-140 (13 micrograms kg-1 intravenous bolus injection, n = 7) and CP-0597 (18 micrograms kg-1 h-1 intravenous infusion, n = 7) on pancreatic microvascular perfusion and leucocyte-endothelium interaction were quantitatively analysed by intravital fluorescence microscopy. Three further groups underwent sham operation (n = 7), ischaemia of 2 h without treatment (n = 7), and ischaemia of 2 h with infusion of phosphate buffer (n = 7). Functional capillary density was significantly greater in animals treated with HOE-140 or CP-0597 than in sham-treated animals, and was decreased to only 60 per cent. Adherence of leucocytes to the endothelium of postcapillary venules was significantly reduced when compared with ischaemia without antagonist. These results demonstrate a positive effect of the two bradykinin antagonists HOE-140 and CP-0597 on microvascular perfusion failure after ischaemia-reperfusion of the pancreas.
对35只麻醉后的斯普拉格-道利大鼠进行胰腺缺血再灌注。通过活体荧光显微镜定量分析两种缓激肽拮抗剂HOE-140（13微克/千克静脉推注，n = 7）和CP-0597（18微克/千克/小时静脉输注，n = 7）对胰腺微血管灌注和白细胞-内皮细胞相互作用的影响。另外三组分别接受假手术（n = 7）、2小时缺血未治疗（n = 7）和2小时缺血并输注磷酸盐缓冲液（n = 7）。用HOE-140或CP-0597治疗的动物的功能性毛细血管密度显著高于假手术组动物，且仅降至60%。与未用拮抗剂的缺血组相比，白细胞与毛细血管后微静脉内皮的黏附显著减少。这些结果表明两种缓激肽拮抗剂HOE-140和CP-0597对胰腺缺血再灌注后的微血管灌注衰竭有积极作用。
