透明质酸从人鼻和气管支气管黏膜下腺的浆液性细胞而非黏液性细胞中胞吐出来。

Hyaluronan is exocytosed from serous, but not mucous cells, of human nasal and tracheobronchial submucosal glands.

作者信息

Baraniuk J N, Shizari T, Sabol M, Ali M, Underhill C B

机构信息

Department of Medicine, Georgetown University, Washington, DC 20007-2197, USA.

出版信息

J Investig Med. 1996 Feb;44(2):47-52.

PMID:8689401

Abstract

BACKGROUND

Hyaluronan is a large, nonsulfated glycosaminoglycan that is a major component of many extracellular matrices. Secretion of hyaluronan has been associated with inflammation in the lungs and other tissues. The purpose of the present study was to determine the distribution of hyaluronan in human respiratory tissues and to determine if hyaluronan was present in human nasal secretions.

METHOD

Hyaluronan was localized in histological sections of human nasal and tracheobronchial mucosa using a biotinlabeled affinity-purified hyaluronan binding probe derived from cartilage proteoglycan (b-PG) (Green et al. Exp Cell Res 1988; 178:224-32). Subjects had saline nasal provocation to collect baseline nasal secretions, then ate chili peppers to provoke parasympathetic cholinergic nasal glandular secretion (gustatory provocation). Total protein and hyaluronan concentrations were measured in the lavage fluids.

RESULTS

Staining for hyaluronan was intense in basement membranes of epithelium, glands and vessels, and perivascular adventitia. Many epithelial cells contained hyaluronan, but the staining intensity was less than that on the basolateral aspects of these cells. Submucosal gland serous cells contained variable amounts of hyaluronan. Hyaluronan was not present in goblet cells, submucosal gland mucous cells, or sub-basement membrane collagen deposits. The gustatory, parasympathetic stimulus induced a 4-fold increase in hyaluronan secretion (P < 0.01) and a 3.5-fold increase in total protein release (P < 0.0010). Hyaluronan accounted for 75% to 80% of the polymeric uronic acids in human nasal secretions.

CONCLUSION

Hyaluronan was present human respiratory epithelial cells and submucosal gland serous cells and was exocytosed in response to parasympathetic stimulation. This distribution suggests roles in packaging cationic proteins in serous cells, cellular adhesion to basement membranes, and activation of macrophages in airway lumens.

摘要

背景

透明质酸是一种大型非硫酸化糖胺聚糖，是许多细胞外基质的主要成分。透明质酸的分泌与肺部及其他组织的炎症有关。本研究的目的是确定透明质酸在人体呼吸道组织中的分布，并确定人体鼻分泌物中是否存在透明质酸。

方法

使用源自软骨蛋白聚糖（b-PG）的生物素标记亲和纯化透明质酸结合探针（Green等人，《实验细胞研究》1988年；178:224-32），将透明质酸定位在人鼻和气管支气管黏膜的组织学切片中。受试者进行盐水滴鼻激发以收集基线鼻分泌物，然后食用辣椒以激发副交感神经胆碱能鼻腺分泌（味觉激发）。测定灌洗液中的总蛋白和透明质酸浓度。

结果

上皮、腺体和血管的基底膜以及血管外膜中透明质酸染色强烈。许多上皮细胞含有透明质酸，但染色强度低于这些细胞基底外侧的染色强度。黏膜下腺浆液细胞含有不同量的透明质酸。杯状细胞、黏膜下腺黏液细胞或基底膜下胶原沉积物中不存在透明质酸。味觉、副交感神经刺激导致透明质酸分泌增加4倍（P<0.01），总蛋白释放增加3.5倍（P<0.0010）。透明质酸占人鼻分泌物中聚合糖醛酸的75%至80%。